As shown in Fig. For multiplexed luminescent and fluorescent assays, using a white plate will support maximum light output signal for the luminescent portion of the assay, but result in higher crosstalk and background for the fluorescent portion of the assay. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Incubate 4h at RT. Incubate 4h at RT. February 23, 2010, WINOOSKI VT, USA - The Synergy TM H4 Hybrid Microplate Reader from BioTek Instruments is now HTRF® certified. For more information. n HTRF plate validated (QC with reference HTRF assays) n Low to high volumes (10 µL to 350 µL) n Can be used with all HTRF compatible readers n For cell-based or biochemical assays Trying is Believing! Plate Characteristics HTRF® 96 - well low volume plate CulturPlateTM 96 - well Microplate CulturPlate TM 384 - well Microplate ProxiPlateTM 384 - Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Lysates are then transferred to the assay plate for the detection of phospho- CHK2 (Thr 68) by HTRF. FOR HTRF CERTIFIED READER For. Fluorescence intensity – Fluorescence intensity is one of the most. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. • ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersFOR HTRF CERTIFIED READER. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Unlike fluorescence intensity which focuses on the quantification of emission intensity at a specific wavelength and neglects its polarization, fluorescence polarization specifically analyses as output the emission intensity of. FOR HTRF CERTIFIED READER . 96-well culture plate• HTRF®-Certified Reader. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. NON-SPECIFIC BINDING TOTAL BINDINGCover the plate with a plate sealer. HTRF reagent concentrations have been set for optimal assay performances. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Highly compatible plates for HTRF. FOR HTRF CERTIFIED READER. The kit includes everything you need: reagents, buffers and a microplate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The FLUOstar Omega is the ideal plate reader for life science applications. FOR HTRF CERTIFIED READER. For a list of HTRF-compatible readers and set-up recommendations, please visit . These convert photons generated by fluorescence or. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Bench top solutions for cell based assays. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Incubate overnight at RT. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. Plus . These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Lysates are then transferred to the assay plate for the detection of total-STING by HTRF. FOR HTRF CERTIFIED READER. For the standard curve described, all wells of the microplate are read. HTRF Signal + 5 hours @ RT or . The CLARIOstar® Plus is our most flexible multi-mode plate reader. culture plate. • HTRF®-Certified Reader **. For more. Add. Discover our high performance reader. Remove the plate sealer and read on an HTRF® compatible reader . For more information about HTRF ®Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Compared to ELISA technology, MSD assays: Require far less sample. It guarantees high level of cAMP accumulation in the cells. For more ® information about HTRF compatible readers and for set-up recommendations, please. Lysates are then transferred to the assay plate for the detection of Total Cofilin by HTRF® reagents. 1. For more information about HTRF. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Incubate overnight at RT. At Cisbio we provide microplates that help you discover better. Lysates are then transferred to the assay plate for the detection of phospho-Tie2 (Tyr992) by HTRF. These plates can be read on all HTRF certified readers, thus making their implementation in. As shown in Fig. FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. Plate 25 μL of cells in. 4 mg/ml. The plate was covered and incubated for two hours at room temperature. 3nm, important when reading at 280 nm for DNA analysis, for more exact results. At Cisbio we provide microplates that help you discover better. Incubate 2 h at RT. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. For more information. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. Certified for use with Alpha, LANCE ® or DELFIA ®. FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. When MikroWin opens, if you already have a pre-existing template for. They can be used in a no-transfer. The Z´ value takes into account four (4) parameters: the means (µ) and the standard deviations (σ) of the positive (plateau) and the negative (baseline) controls 1. Partnerships for delivering turn-key solutions. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. FOR READING, AN HTRF ®-CERTIFIED READER IS NEEDED. Dedicated UV-laser excitation. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYDetection. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. HTRF Signal + 16 µL 4 µL Standard of pre-mixed IL8 Antibodies or Sample ADD READ ANALYSE Incubate 1 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed IL1β Antibodies ADD READ ANALYSE Incubate overnight* at room temperature *Reading possibe after 2h White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. VICTOR® Nivo™ multimode plate reader 2 | For research use only. STANDARD PROTOCOL FOR INHIBITORY ASSAY IN 20 μL FINAL VOLUME Standard Inhibitor Tag1-TNFα Tag2-TNFR1 Anti-Tag1 Eu Cryptate antibody Anti-Tag2 XL665 reagent PPI Europium detection bufferThe perfect microplate reader for everyday biochemical and cell-based assays. FOR HTRF CERTIFIED READER. Please note that this kit use a Terbium donor crypate, make sure to set up your reader for Terbium cryptate. As shown in Fig. CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. Plate 25 μL of cells in. use of a wide variety of plates or sample matrix formats. The ratio of the two fluorescence Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to. + 4 µL HTRF pre-mixed antibodies. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. Plates can be loaded manually or plates can be loaded using 20 or 50-plate free access magazines which fit in a stacker. Provide more sensitive and precise results. Plate 25 μL of cells in. 10 µL Standard or Sample. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The samples were each placed in a well of a 24-well plate, covered with 1 ml of culture medium, and incubated for a total of 17 days. spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf certifiedLysates are then transferred ®to the assay plate for the detection of Total CHK2 by HTRF reagents. EnSight ® offers both fast well-imaging and multimode detection capabilities for target-based and phenotypic screening, all in a single benchtop instrument. Cover the plate with a plate sealer. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Incubate overnight at RT. • User-upgradable microplate reader in under 2 minutes • Array of detection cartridge applications at your fingertips. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. (665 nm and 620 nm) on a compatible HTRF ® reader. and Canaa -8-963-4567. Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. Choosing the right microplate reader ensures you’ll get an optimal readout. Results for HTRF Reader Control Kit with black and white microplates. FOR HTRF CERTIFIED READER culture plate to a 384-well small volume white plate. Make sure the setup for Tb Cryptate is used. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-2 (Ser473) by HTRF. Certified HTRF compatible reader by CisBio Bioassays;Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. If not, our scientific team will help you choose the instrument that best suits your needs and budget. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. For more information about HTRF. 96-well culture plate+ 4 µL HTRF pre-mixed antibodies. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Lysates are then transferred to the assay plate for the detection of total-CREB by HTRF. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. Customer Transformation Hub. FOR HTRF CERTIFIED READER. The kit includes everything you need: reagents, buffers and a microplate. Better sensitivity provides better statistic, less variability among replicates, and a larger assay window. Each well generally requires 50µL of lysis buffer 1X. (donor) of compounds are measured on an HTRF-compatible reader. The kit includes reagents, buffers and a microplate. • Clone your plate data for multiple data reduction optionsExpanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. The Dual-Luciferase ® Reporter Assay or DLR is widely used to study gene transcription and regulation. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readers HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. The FLUOstar ® Omega is a multi-mode microplate reader with three detection modes. Phospho-Rb (Ser780) two-plate assay protocol. FOR HTRF CERTIFIED READERThis makes the PHERAstar ®FSX the fastest laser-based TRF reader on the market: 8 seconds for a 96-well plate. Incubate overnight at RT. It can also be further streamlinedFOR HTRF CERTIFIED READER. For more information about HTRF ®The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. " The PHERAstar FSX instrument is a high quality, reliable, plate reader. HTRF is compatible with a large range of available microplate readers. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Remove the plate seal and read on an HTRF compatible reader. 10 μL of GLP1 receptor cells were dispensed into wells of. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Lysates are then transferred to the assay plate for the detection of Total H2AX by HTRF ® reagents. The PHERAstar ® FSX high-throughput screening microplate reader uses unique, easy-to-configure optic modules. no. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Place the imaging filter cube on top of the LED cube you installed, and use the hex wrench to screw it to the LED cube. FOR HTRF CERTIFIED READER. Specialized filters allow for IR fluorescence. The signals are produced by, converted by, or transmitted through the. The dual measurement. Read & analyse. These are the different kinetic modes on BMG LABTECH microplate readers. * For HTRF microplate recommendations, please visit Table 1 Instrument settings used for HTRF with terbium donor and red acceptor. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. 5 EXAMPLE OF MAP PLATE . The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. ® Detection of Progesterone Receptor with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Up to 8 decades dynamic range in one measurement with EDR technology. FOR HTRF CERTIFIED READER. Read & analyse. Tecan Spark multimode microplate reader, with fluorescence Fusion Optics™ meets these challenges and offers everything you need to develop and run advanced biochemical and cell-based fluorescence assays. FOR HTRF CERTIFIED READER . Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. For more information. Assay plate setup. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Detection plates from providers not listed on this. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. This protocol, HTS designed, allows. Plus . FOR HTRF CERTIFIED. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Rating: 5. The plate was covered and incubated for one hour at room temperature. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Make sure the setup for Tb Cryptate is used. The VICTOR Nivo is a HTRF certified microplate reader. No washing steps are required. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. 25,000 human Jurkat cells were plated in 96 well plates. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 4. For more information about HTRF. For more information about HTRF. It can also be further streamlined to a one-. Fluorescence intensity – Fluorescence intensity is one of the most popular detection modes, and there are innumerable fluorophores available for applications such as DNA or protein quantitation, reporter-geneCertified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. The 2 plate protocol involves culturing cells in a 96-well plate before lysis then transferring lysates to a 384-well low volume detection plate before adding Phospho-ERK (Thr202/Tyr204) HTRF detection reagents. High Speed Monochromator offers a typical wavelength accuracy of <0. For a list of HTRF-compatible readers and set-up recommendations, please visit . HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed TNFα Antibodies ADD READ ANALYSE Incubate 2 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. 5 µL Prostaglandin E2 . It combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background. FOR HTRF CERTIFIED READER. It can also be further streamlined toFOR HTRF CERTIFIED READER. . The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Measure elevated and normal expression samples without multiple dilutions. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". FOR HTRF CERTIFIED READER. Lyse cells Read on an HTRF compatible reader . Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. This protocol, HTS designed, allows miniaturization while. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-3 (Ser473) by HTRF. Set up your reader for Eu3+ Cryptate and read the fluorescence emission at two different wavelengths (665nm and 620nm) on a compatible HTRF® reader**. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. PerkinElmer’s 96w low volume, 96w and 384w microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Flexibility combined with the best sensitivity of its class, and our newly developed Enhanced Dynamic Range technology, make it the ideal reader for assay development. -Certified Reader** *Assay volumes can be adjusted proportionally to run the assay in 96- or 1536-well microplates. Conclusion. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Lysates are then transferred to the assay plate for the detection of phospho-c-Src (Tyr419) by HTRF. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. Finding a plate reader that offers compatibility with microplates sold by multiple leading vendors can protect both the long-term cost and the performance of assays. CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . Download the brochure to learn how this compact and robust device can handle various detection modes, plate formats, and software options. FOR HTRF CERTIFIED. Example assays are ELISAs, DNA and protein concentration determination, steady-state dose response curves, ADP-Glo™ (Promega), Transcreener™ GDP fluorescent polarization. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Read & analyse. * For HTRF microplate recommendations, please visit . • HTRF®-Certified Reader. Plate cells Stimulate cells 3. • HTRF®-Certified Reader. The EnSight<sup>®</sup> multimode plate reader offers HTS Alpha and Ultra-sensitive Luminescence technologies alongside conventional multimode detection technologies. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Read & analyse. HTRF-compatible SpectraMax Multi-Mode Readers. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. MATERIALS PROVIDED: KIT . Revvity. Cells in each image are identified by SoftMax Pro Software and cell-by-cell HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. Neocarzinostatin dose-response on Jurkat cells. Microplate readerFluorescence microplate readers usually contain a light source, an optical system for the selection of excitation light (i. Incubate 4h at RT. Read & analyse. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. + 4 µL HTRF pre-mixed antibodies. 7ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED. For more information about HTRF. Incubate overnight at RT. It can also be further streamlined toLysates are then transferred ®to the assay plate for the detection of Total TYK2 by HTRF reagents. For more information about HTRF. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. -Certified Reader** *Assay volumes can be adjusted proportionally to run the assay in 96- or 1536-well microplates. Read & analyse. Experimental details• HTRF®-Certified Reader **. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the. Incubate 4h at RT. This protocol, HTS designed, allows miniaturization while. It also integrates with the powerful SoftMax Pro Software for. The HTRF cellular assays were found to be at least 16-fold more sensitive than the Western Blot. Read & analyse. The controls are equivalent to the upper and lower limit of. For more information about HTRF. Lysates are then transferred to the assay plate for the detection of Phospho-VASP (Ser239) by HTRF. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Assay setup for a 384-well low-volume plate. For HTRF certified reader For more information about HTRF® compatible readers and for set- up recommendations, please visit our website. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF® pre-mixed antibodies Read & analyse Incubate 4h at RT Transfer 16 µL supernatant LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . Learn about the principles, applications, and benefits of HTRF in this Agilent application note. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Transcreener® ADP2 TR-FRET assay. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Read & analyse. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. It fits any benchtop – and budget Building on our pioneering family of benchtop multimode plate readers, the VICTOR® Nivo™ multimode reader breaks new ground, packing all popular detection modes into the industry’s smallest benchtop. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. PURCHASE SEPARATELY . ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. Introduction. The HTRF ratio obtained represents the total binding. * For HTRF microplate recommendations, please visit . The plate is set up for reading and images are acquired according to specified parameters. This DLReady-certified microplate reader can also come equipped with Simultaneous Dual Emission for BRET applications and can be further upgraded to a multi-mode reader. This protocol gives the cells viability and confluence to be monitored. Dedicated UV-laser excitation. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. For more information about HTRF ®Excitation range. . These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. NanoQuant Plate™ allows rapid, straightforward measurement of 16 x 2 µl samples simultaneously without calibration. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. For more. For HTRF certified readerThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Even if not listed, some readers may be compatible with HTRF technology. FOR HTRF CERTIFIED READER. FLUOstar Omega Filter-based multi-mode microplate reader . The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The tried, tested, and proven EnVision plate reader has an array of exciting features that can enhance your applications. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. FOR HTRF CERTIFIED READER For more. SV detection plate. Homogeneous Time-Resolved Fluorescence (HTRF) . BMG LABTECH’s UV-laser not only allows for faster read times in europium TRF assays, but it also greatly enhances performance in terbium-based TRF assays. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate AntibodyCisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. For more ® information about HTRF compatible readers and for set-up recommendations, please. Plate 25 μL of cells in. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate Antibody• HTRF®-Certified Reader. 5% and an increased Z’ value of 0. The lysis buffer 4X is ready-to-use. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. Discover the features and benefits of the VICTOR Nivo multimode plate reader, the smallest and most versatile instrument for life science applications. Reagents were dispensed following Cisbio’s ligand binding protocol (Figure 3). Plate 25 μL of cells in. FOR HTRF CERTIFIED READER. In particular, these parameters should be entered as defined in the table below Setup Top filter cube EX 330 / LUM (block #18) EM 620 / 665 / LUM (block #41) Light source LaserDetect flash, glow, Dual Luciferase ® Reporter and BRET assays. Both readers demonstrated their ability to perform the cAMP G s HiRange assay with results well within Cisbio’s required. Reader. 96-well or 384-well small volume (SV) detection microplates - For more information about microplate recommendations, please. It measures 96-well plates in less than 20 seconds and 1536-well formats in under one minute. FOR HTRF CERTIFIED READER For. FOR HTRF CERTIFIED READER. + 4 µL HTRF ® pre-mixed antibodies .